RASSF3 Polyclonal Antibody

Catalogue Numbers: BS60826-50, BS60826-100

Sizes: 50µl, 100µl

Alternative Name: Ras association domain-containing protein 3; RASSF3

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q86WH2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Ras is a small GTP-binding protein involved in many cellular processes, including proliferation, differentiation and apoptosis. Ras transmits signals of cell surface receptors by binding to a variety of effector molecules. In addition to the well characterized effectors Raf and PI 3-kinase, Ras also interacts with members of the RASSF family, including RASSF1, RASSF2, RASSF3, RASSF4 and Nore1. Members of the RASSF family contain a highly conserved Ras association domain (Ral GDS/AF-6 or RA) and function as Ras effectors/tumor suppressors. RASSF3 (Ras association domain family 3) is a ubiquitously expressed protein found in normal and cancerous tissues. RASSF3 contains an N-terminal RA domain and a coiled-coil SARAH domain. Three isoforms, namely RASSF3A, RASSF3B and RASSF3C, may exist for RASSF3 due to alternative splicing. RASSF3B and RASSF3C are shorter than RASSF3A and do not contain the RA and SARAH domain.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RASSF3 polyclonal antibody detects endogenous levels of RASSF3 protein.

Bioworld Molecular Weight: ~ 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RASSF3 polyclonal antibody at 1:500 dilution
Lane 1: Mcf-7 whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human RASSF3.

Conjugate: Unconjugated

Modification: Unmodified