General Information

• Product Name: α-Chymotrypsin
• Enzyme Commission (EC) Number: 3.4.21.1
• Source: Typically derived from bovine pancreas (Bos taurus)
• CAS Number: 9004-07-3
• Molecular Formula: Protein (Enzyme)
• Molecular Weight: ~25 kDa

Physical and Chemical Characteristics

• Appearance: White to light beige lyophilized powder
• Solubility: Soluble in water and buffered solutions, slightly soluble in organic solvents
• Optimum pH: 7.5-8.5
• Optimum Temperature: 37°C
• Isoelectric Point (pI): ~8.8

Specifications

• Enzyme Activity: ≥60 U/mg (1 unit hydrolyzes 1.0 µmol of N-glutaryl-L-phenylalanine p-nitroanilide per minute at pH 7.6, 25°C)
• Purity: ≥90% by protein content
• Moisture Content: ≤5.0%
• Heavy Metals: ≤10 ppm
• Microbial Contamination: ≤100 CFU/g
• Protease Inhibitor Sensitivity: Inhibited by PMSF (phenylmethylsulfonyl fluoride)

Stability and Storage

• Stability: Stable for at least 2 years when stored properly
• Storage Conditions: Store at -20°C for long-term stability; protect from moisture and light
• Reconstitution Stability: Once reconstituted, store at 2-8°C and use within a few days

Handling and Safety

• Handling: Avoid direct contact; use in a well-ventilated area
• Safety Precautions: Wear protective gloves, safety glasses, and a dust mask when handling
• Disposal: Dispose of according to local environmental regulations and guidelines

Regulatory Compliance

• Biochemical Grade: Suitable for research and industrial applications
• Pharmaceutical Grade: Compliant with USP and EP standards (if applicable)
• ISO Certification: Compliant with International Organization for Standardization (ISO) standards

Packaging

• Packaging Size: 1g
• Packaging Material: Airtight vials or sealed containers
• Labeling: Includes product name, enzyme activity, molecular weight, CAS number, and batch number

Applications

• Biochemical Research: Used for protein digestion in proteomics and enzymatic studies
• Pharmaceutical Industry: Applied in wound debridement and enzymatic therapy
• Food Industry: Utilized in food processing, tenderizing proteins, and flavor enhancement
• Biotechnology: Used in cell culture applications and recombinant protein processing