Tropomyosin 3 Monoclonal Antibody

Catalogue Numbers: MB66819-50, MB66819-100

Sizes: 50µl, 100µl

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P06753

Host: Mouse

Reactivity: Human, Mouse

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Tropomyosins are a group of structural proteins. Tropomyosins are present in virtually all eukaryotic cells, both muscle and non-muscle, where they bind Actin filaments and function to modulate Actin-Myosin interaction and stabilize Actin filament structure. Tropomyosin a is encoded by the TPM1 gene, which maps to human chromosome 15q22.2 and undergoes alternative splicing to
generate at least four isoforms, including skeletal muscle (isoform 1), smooth muscle (isoform 2), fibroblast/TM3 (isoform 3) and isoform 4. Tropomyosin b is encoded by the TPM2 gene, which maps to human chromosome 9p13.3 and undergoes alternative splicing to generate three isoforms, including skeletal muscle (isoform 1), non-muscle/fibroblast TM36/epithelial TMe1 (isoform 2) and non-muscle (isoform 3). Troponin I binds Tropomyosin at a specific region and the association of Tropomyosin-Troponin with Actin filaments may increase the rigidity of Actin filaments. Tropomyosin also interacts with Caldesmon to regulate smooth muscle contraction.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Tropomyosin 3 protein.

Extra Notes: Western blot analysis of Tropomyosin 3 expression in Hela (A), NIH3T3 (B) whole cell lysates.

Bioworld Molecular Weight: ~ 29 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Tropomyosin alpha-3 chain; Gamma-tropomyosin; Tropomyosin-3; Tropomyosin-5; hTM5

Immunogen: Recombinant fusion protein of human Tropomyosin 3. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified