mAChR M2 (P224) Polyclonal Antibody

Catalogue Numbers: BS2781-50, BS2781-100

Sizes: 50µl, 100µl

Alternative Name: CHRM2; Muscarinic acetylcholine receptor M2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08172

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: The muscarinic acetylcholine receptors (mAChR) mediate a variety of cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels. The mAChRs transducer signals by coupling to G-proteins, which then modulate several downstream effector proteins and ion channels. Five mAChR subtypes have been identified, designated M1 to M5. The five receptor subtypes show distinct patterns of tissue distribution, as well as distinct pharmacological and functional properties.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: mAChR M2 (P224) polyclonal antibody detects endogenous levels of mAChR M2 protein.

Bioworld Molecular Weight: ~ 52 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of mAChR M2 (P224) pAb at 1:1000 dilution
Lane 1: CT26 whole cell lysate(40ug)
Lane 2: PC12 whole cell lysate(40ug)
Lane 3: Hela whole cell lysate(40ug)
Lane 4: SGC7901 whole cell lysate(40ug)
Lane 5: HEK293T whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 191-240 of Human mAChR M2.

Conjugate: Unconjugated

Modification: Unmodified