RPA4 Polyclonal Antibody

Catalogue Numbers: BS60574-50, BS60574-100

Sizes: 50µl, 100µl

Alternative Name: Replication protein A 30 kDa subunit; RP-A p30; Replication factor A protein 4; RF-A protein 4; RPA4

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13156

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB consisting of three subunits that play an important role in DNA replication, recombination and repair. RPA is one of the major damage- recognition structures involved in the early stage of nucleotide excision repair and may function in telomere maintenance. The binding of human RPA (hRPA) to DNA involves molecular polarity, in which initial hRPA binding occurs on the 5' side of a ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. Widely expressed at low to intermediate levels,RPA 34 kDa subunit, which is also known as RPA4 (replication factor A protein 4), contains 261 amino acids, localizes to nucleus and is preferentially
expressed in mucosa of colon and placenta.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RPA4 polyclonal antibody detects endogenous levels of RPA4 protein.

Bioworld Molecular Weight: ~ 29 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RPA4 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human RPA4

Conjugate: Unconjugated

Modification: Unmodified