XBP1 Rabbit Monoclonal Antibody

Catalogue Numbers: MB66465-50, MB66465-100

Sizes: 50µl, 100µl

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Swiss-Prot: P17861

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IP

All Applications: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Background: Following protein synthesis, secretory, intra-organellar, and transmembrane proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. The accumulation of unfolded proteins within the ER triggers an adaptive mechanism known as the unfolded protein response (UPR) that counteracts compromised protein folding. The transmembrane serine/threonine kinase IRE1, originally identified in Saccharomyces cerevisiae, is a proximal sensor for the UPR that transmits the unfolded protein signal across the ER membrane. The human homolog IRE1α was later identified and is ubiquitously expressed in human tissues. Upon activation of the unfolded protein response, IRE1α splices X-box binding protein 1 (XBP-1) mRNA through an unconventional mechanism using its endoribonuclease activity. This reaction converts XBP-1 from an unspliced XBP-1u isoform to the spliced XBP-1s isoform, which is a potent transcriptional activator that induces expression of many UPR responsive genes.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of XBP1 protein.

Extra Notes: Western blot analysis of XBP1 expression in Raw264.7 (A), HL60 (B), U2OS (C) whole cell lysates.

Bioworld Molecular Weight: ~ 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: TREB5; XBP2; X-box-binding protein 1; XBP-1; Tax-responsive element-binding protein 5

Immunogen: Recombinant protein of human XBP1

Conjugate: Unconjugated

Modification: Unmodified