FHL3 Polyclonal Antibody

Catalogue Numbers: BS60054-50, BS60054-100

Sizes: 50µl, 100µl

Alternative Name: Four and a half LIM domains protein 3; FHL-3; Skeletal muscle LIM-protein 2; SLIM-2; SLIM2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13643

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The four-and-a-half-LIM domain (FHL) proteins include FHL-1 (SLIM1), FHL-2 (SLIM3), FHL-3 (SLIM2) and FHL-4. The signature “half-domain”, a single zinc finger domain located in the N-terminal region, differentiates FHLs from other LIM-only proteins, which have numbers of zinc fingers. Specific combinations of FHL proteins elicit selective activation of both CREB and CREM. Skeletal and cardiac muscle express FHL-1 in high levels as compared to the low level of expression in smooth muscle of the colon, small intestine and prostate. FHL-1 localizes to the cytosol of myoblasts, myotubes, and differentiated myocytes. FHL-2 is also located in cardiac and skeletal muscle, as well as in placenta and ovary tissues. FHL-3 is found in skeletal muscle, but absent in cardiac muscle. FHL-4 is expressed exclusively by the seminiferous epithelium of the testis, which suggests that FHL-4 is involved in spermatogenesis. The genetic loci for FHLs vary considerably despite similiar amino acid sequences among the FHL group.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: FHL3 polyclonal antibody detects endogenous levels of FHL3 protein.

Bioworld Molecular Weight: ~ 31 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FHL3 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human FHL3.

Conjugate: Unconjugated

Modification: Unmodified