MYLK Polyclonal Antibody

Catalogue Numbers: BS5809-50, BS5809-100

Sizes: 50µl, 100µl

Alternative Name: Myosin light chain kinase, smooth muscle; MLCK; smMLCK; Kinase-related protein; KRP; Telokin; Myosin light chain kinase, smooth muscle, deglutamylated form; MLCK; MLCK1; MYLK1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15746

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and myosin light chain kinases (MYLK, also designated MLCK). The MYLK kinases phosphorylate myosin regulatory light chains to catalyze myosin interaction with actin filaments resulting in contractile activity. Non-muscle, smooth muscle and skeletal/cardiac muscle MYLK isoforms exist. The MYLK gene (also designated MYLK1) encodes both smooth muscle and non-muscle isoforms as well as telokin, a small C-terminal isoform expressed only in smooth muscle with the capacity to stabilize unphosphorylated myosin filaments. Multiple transcript variants are described for the MYLK gene. Smooth-muscle and non-muscle MYLK isoforms are expressed in a wide variety of adult and fetal tissues. The skeletal/cardiac muscle isoforms of MYLK are encoded by a separate gene, MYLK2 (also designated skMLCK). MYLK appears to be a target for PAKs (p21-activated kinases). PAK1 interaction with MYLK results in a decrease in MYLK activity and myosin light chain phosphorylation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MYLK polyclonal antibody detects endogenous levels of MYLK protein.

Bioworld Molecular Weight: ~ 135, 210 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MYLK Antibody at 1:500 dilution
Lane 1: A375 whole cell lysate(40ug)
Lane 2: HCT116 whole cell lysate(40ug)
Lane 3: MCF-7 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 1750-1795 of Human MYLK.

Conjugate: Unconjugated

Modification: Unmodified