HES-2 Polyclonal Antibody

Catalogue Numbers: BS60286-50, BS60286-100

Sizes: 50µl, 100µl

Alternative Name: Transcription factor HES-2; Class B basic helix-loop-helix protein 40; bHLHb40; Hairy and enhancer of split 2; HES2; BHLHB40

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y543

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues. HES3 is expressed exclusively in cerebellar Purkinje cells, and HES5 is found solely in the nervous system. HES6 is produced in brain as well as in the limb buds of developing embryos.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HES-2 polyclonal antibody detects endogenous levels of HES-2 protein.

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HES-2 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate

Immunogen: A synthetic peptide corresponding to residues in Human HES-2.

Conjugate: Unconjugated

Modification: Unmodified