GLK (G4) Polyclonal Antibody

Catalogue Numbers: BS2755-50, BS2755-100

Sizes: 50µl, 100µl

Alternative Name: Mitogen-activated protein kinase kinase kinase kinase 3; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; Germinal center kinase related protein kinase; MEKKK 3; MAP4K3

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8IVH8

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Several mammalian kinases have been identified which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include GLK, KHS, NIK, YSK1, HPK1, Krs-1, Krs-2 and human GC kinase. GLK (for GC-like kinase) is an 885 amino acid protein that shares a high degree of homology with GC kinase and HPK1. Like many of the STE20-like kinases, GLK specifically activates
the JNK pathway. Epistasis studies with dominant negative mutants of MEKK1 suggest that GLK functions upstream of MEKK1 in the JNK signaling pathway.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GLK (G4) polyclonal antibody detects endogenous levels of GLK protein.

Bioworld Molecular Weight: ~ 65 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GLK (G4) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human GLK.

Conjugate: Unconjugated

Modification: Unmodified