GAPDHS Polyclonal Antibody

Catalogue Numbers: BS60630-50, BS60630-100

Sizes: 50µl, 100µl

Alternative Name: Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; GAPDH-2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; GAPDHS; GAPD2; GAPDH2; GAPDS; HSD-35; HSD35

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14556

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also called uracil DNA glycosylase, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. While GAPDH has long been recognized as playing an integral role in glycolysis, additional functions of GAPDH include acting as a uricil DNA glycosylase, activating transcription, binding RNA and involvement in nuclear RNA export, DNA replication and DNA repair. Expression of GAPDH is upregulated in liver, lung and prostate cancers. GAPDH translocates to the nucleus during apoptosis. GAPDH complexes with neuronal proteins implicated in human neuro-degenerative disorders including the β-Amyloid precursor, Huntingtin and other triplet repeat neuronal disorder proteins.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GAPDHS polyclonal antibody detects endogenous levels of GAPDHS protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GAPDHS polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: pc12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human GAPDHS

Conjugate: Unconjugated

Modification: Unmodified